279 resultados para bioscience

em Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho"


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The contribution of green manure to soil improvement and crop production depends primarily on biomass production and its chemical composition, which vary depending on the species, region and growing season. The aim of this research was to evaluate the chemical composition of biomass produced by green manures in Vale do Ribeira, São Paulo, Brazil. In order to develop this research, was carried an experiment in Pariquera-Acu, in 2006/2007, in completely randomized blocks design with four treatments (three green manure and spontaneous vegetation) and five replications. At 30, 60, 90 and 120 after sowing samples were collected in 1m(2) of the shoots and determined fresh and dry, and chemical composition biomass. Sunhemp, pigeon pea and mucuna produced, in decreasing order, the largest quantities of biomass and were more efficient than the spontaneous vegetation. The biomass produced by green manure had higher quality than that produced by spontaneous vegetation. Sunhemp and pigeon pea have a higher proportion of dry matter in stems which have low N, high C/N and L/N ratio, variables indicating slow decomposition of residues. The analysis of dry matter partitioned to better indication of the chemical composition of the residues and the prevision of the availability of nutrients in the soil.

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The soil resistance to penetration and shear can be used as indicators of soil compaction and to indicate the susceptibility of a soil to erosion. The objective of this study was to quantify and compare the impact provided by different land uses in a haplic cambissol in areas of permanent preservation, from the soil resistance to penetration and shear. The experimental area was located in an area of permanent preservation, the sub-basin of the Ribeira Iguape River - SP, with different land uses: banana cultivation - CBAN, degraded pasture - PDEG, silvopastoral system - MPIS and native forest - MNAT. The test for resistance to penetration was accomplished with a digital penetrometer compaction of manual effort, to a depth of 40 cm. The soil shear strength was determined by Vane Test at a depth between 0 and 5 cm. The degraded pasture was similar to native forest, with less resistance to penetration. The banana cultivation and silvopastoral system were the land uses with the highest resistance to penetration, bringing serious risk of erosion in areas of permanent preservation. The soil under native forest had lower shear strength. The cultivation of bananas, degraded pasture and silvopastoral system were the land uses with higher shear strength of soil, indicating that the use of these soils in areas of permanent preservation is promoting the same compression.

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This work was divided in two experiments, the first one to evaluate the lettuce productivity (cv Lucy Brown - American group) influenced by increasing and decreasing periods of coexisting with harmful plants, and a second moment, aiming at to evaluate the allelopathic potential of nutsedge (Cyperus rotundus L.) on the initial lettuce development.The experimental treatments constituted of six increasing and decreasing periods of coexisting with harmful plants, or control of it in the culture, considered from the plantation of the lettuce; being separate in two groups: Weeds and Clean weeds.In the second moment of the experiment, purple nutsedge aerial parts had been collected in the area of experiment 1, that drying had been after triturated and immersed in methylic alcohol P. A in ratios 10, 5,0 and 2.5% (w/ v) and later impregnated in germination paper, where had been placed lettuce seeds.The analyses of results, one concludes that the presence of C. rotundus plants can intervene with the germination, growth and development of the plants of lettuce cv Lucy Brown, and that initial periods of coexisting between the culture and the harmful are associate to an induction of the foliar area development, and can not express the reduction of productivity in the end of the cycle. Drawn out periods of competition induce the reduction of the lettuce foliar area, and then they will intervene with the yield productivity.

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The objective of this work was to evaluate Cd, Cr, Ni and Pb total contents in soil and sugar cane after four annual applications of sewage sludge and vinasse to supply all N and all K required by the crop. A field experiment was carried out on clayey Typic Haplustox. It was used a randomized block design with seven treatments and three replications. The treatments consisted in: (i) rate of sewage sludge to supply 100% of the N required by the crop; (ii) rate of sewage sludge to supply 200% of the N required; (iii) rate of vinasse to supply 100% of the K required; (iv) rate of vinasse to supply 200% of the K required; ( v) combination of the treatments ( i) and ( iii); ( vi) combination of the treatments ( ii) e ( iv); (vii) control ( mineral fertilization). The contents of Cd, Cr, Ni and Pb in sewage sludge reached maximum values of 1.58, 41.66, 35.17 and 45.25 mg kg(-1), respectively. These metals were not detected in vinasse. The residues were applied annually for four years. At the end of this period, sewage sludge and vinasse accumulated rates, that get up to 51 t ha(-1) and 762 m(3) ha(-1), respectively, did not cause alteration in Cd, Cr, Ni and Pb total contents in soil, up to 50 cm of depth, and in plant. Sewage sludge and vinasse application to supply N and K for sugar cane did not offer soil pollution risk by heavy metals.

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With the purpose to study the emergence of Cenchrus echinatus L. and Cosmos sulphureus Cav. in response to time and depth of sowing, two experiments were conducted in uncontrolled environmental conditions in the months of June and September 2009. Cenchrus echinatus L. and Cosmos sulphureus Cav seeds were sown at different depths (0, 1, 3, 5, 7 and 9 cm). The experiments were conducted in pots, with five replications of 50 seeds per pot, followed by evaluation of the emergence percentage and emergence speed index (ESI). The highest percentage of emergency and higher EVR were observed up to 5 cm depth and the species Cosmos sulphureus Cav. was more sensitive to temperature variations due to the time of sowing of the species C. equinatus.

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The major problems in pasture areas are weed management. Sida rhombifolia L. is a major weed of these areas, causing serious problems for the farmers. This research aimed to understand the importance of Sida rhombifolia L. seed depth in the soil profile to increase the germination rate and seedling emergence potential. During the month of September 2008 (spring) and January 2009 (summer), two assays were carried out under greenhouse conditions and the seeds were sown at 0, 1, 2, 3, 4, and 5 cm from the soil surface. The experiment was conducted in pots, 5 L, with 5 replicates containing 50 seeds per pot. In both seasons, the emergence percentage and germination speed were increasing until the 17th day after sowing, and the depths of one, two and three centimeters were those which obtained higher emergencies and germination speed. In one cm depth, the germination was 28% and 32% in spring and summer, respectively, statistically different from the other depths. however, five depths centimeters; the germination was only 8% and 13% respectively.

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Knowledge of the leaf area plant are needed for agronomic and physiological studies involving plant growth. The aim of this study was to obtain a mathematical model using linear measures of leaf dimensions, which will allow the estimation of leaf area of Crotalaria juncea L. Correlation studies were conducted involving real leaf area (Sf) and leaf length (C), maximum leaf width (L) and the product between C and L. All tested models (linear, exponential or geometric) provided good estimation of leaf area (above 87%). The better fit was attained using linear model, passing or not through the origin. From a practical viewpoint, it is suggested to use the linear model involving the C and L product, using a linear coefficient equal to zero. Estimation of leaf area of Crotalaria juncea L. can be obtained using the model Sf = 0.7160 x (C*L) with a determination coefficient of 0.9712.

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This experiment were performed to evaluate methods of seeds scarification for overcoming dormancy and the efficacy of six herbicides registered for sugarcane to control adult plants of Mcharantia. The experimental design was completely randomized, with four replications in both trials. The first trial is a factorial 2x8 (two times of seeds collection (2006 and 2007) with eight methods of breaking dormancy (mechanical scarification; 100% and 50% concentrated sulfuric acid; 2% potassium nitrate for three and six hours; heat dried at 60 degrees C; hot water at 60 degrees C and an untreated control). In the second trial evaluated the control of M. charantia through six different herbicides: Imazapic, Metsulfuron-methyl, Metribuzin, 2,4-D, Amicarbazone, Paraquat and a control without herbicide application. Thus, it can be concluded that the seeds were immersed in concentrated sulfuric acid for three minutes and potassium nitrate for three hours gave a higher percentage and germination speed and the herbicide Metribuzin reached, albeit belatedly, a total control of M. charantia.

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The purpose of this study was to evaluate germination of Momordica charantia L. seeds under different conditions of: water availability (0.0, -0.2, -0.4, -0.6, -0.8 and -1.0 MPa), temperature (10, 15, 20, 25, 30 and 35 degrees C) and quality of incident light (filter dark, red, green, yellow, red off, blue, orange and without filter). For each variable evaluated, experiments were carried out in a completely randomized design with four replicates kept under controlled conditions (germination chamber). There were higher percentages of germination under conditions of mild water restriction (-0.2 MPa) and temperatures around 25 degrees C, regardless of the quality of light. Temperatures below 20 degrees C and above 30 degrees C reduced germination, although lower temperatures had strongly effects on germination. Water availability lower than -0.2 MPa strongly reduced germination, which was inhibited in the treatments of -0.8 and 1.0 MPa.

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The researches on the biological control of nematodes with nematophagous fungi has been intensified in recent years. The knowledge of the ecological conditions for the growth and sporulation of these fungi is a prerequisite for attainment of pure cultures needed to attend the demand for formulation of these organisms. With the objective to evaluate the micelial growth and sporulation of Arthrobotrys musiformis and A. oligospora in two environments (B.O.D at 25 +/- 1 degrees C and the environment of the Laboratory), 20 cultures media prepared with common materials found in the communities and industrialized media such as mycological agar, PDA and CMA were evaluated. The media were tested in Petri dishes, being the micelial growth of the fungi evaluated daily, during six days. The measured sporulation at the end of the experiment was done by estimation of the number of conidia/Petri dish. The experiment was carried out in a random design following a factorial arrangement of 20 x 2 x 2, corresponding to 20 media, two fungi and two environments, with five replicates. The variance analysis of the data evidenced significant statistical difference by the F Test, at 1% probability, among media x fungi x environment interaction. Fifty percent of the tested media provided the adequate micelial growth of A. musiformis and there was no statistical difference among them, namely: cassava meal (FM), sweet starch (PD), "corn meal agar" (CMA), oat in fine flakes (AFF), agar-water + dextrose (AA+D), mycological agar (AM), potato dextrose agar (BDA), meal of maize (FMI), flour of wheat (FT) and wheat for kibble (TK). In relation to A. oligospora, 75% of the tested media promoted the maximum growth of the fungus, which are: AFF, AM, FM, PD, CMA, AA+D, BDA, FT, TK, the water from the decoction of rice (AAZ), rice in grains (AZG), triturated rice (AZT), thread flour (FR), oats flour (FA), oats in thick flakes (AFG) and flour of maize (FU). In relation to the sporulation the media that had better role for A. musiformis, in decreasing order, were: FR, TK, AFG, BDA, FA, AFF, AM, FMI, AZT and FM, varying between 1,01 x 10(6) and 1,4 x 10(4) conidia/Petri dish. For the A. oligospora sporulation, the CMA medium provided the maximum level with an estimated average of 5,7 x 10(6) conidia/Petri dish. In the general, the best media for the micelial growth and sporulation of A. musiformis had also been the best for A. oligospora. However, some that had been the best for the A. oligospora did not had been efficient for the micelial growth or the sporulation of A. musiformis, indicating that the isolate of A. musiformis in case is more demanding than that A. oligospora one. The evidences from the study indicate that, in Jaboticabal, São Paulo state, the growth and the sporulation of these fungi do not demand special chambers. Some adaptations of an environment at the laboratory, enough to obliterate the light are sufficient.

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Three methods of sexual identification were evaluated in Nile tilapia (Oreochromis niloticus) at ages of 30, 60 and 90 days that were submitted to sexual reversion under administration of androgen hormone 17 alpha-methyltestosterone added to crumble diets with different granules sizes (0.25, 0.35 and 0.50 mm) in dosage of 60mg/kg. The techniques of sexual identification employed were: a) Macroscopic examination of urogenital papilla; b) Microscopic examination of gonads through stain with carmine-acetate and c) Microscopic examination of gonads through histological routine. In the presence of three methods of sexual identification evaluated, histology of gonads has shown the most confident diagnosis. The grain diameters of rations did not interfere in morphological characteristics of gonads, neither in secondary sexual characters of fish.

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It was evaluated the intercropping of lettuce groups economically with roquette, in two experiments, it was carried at Unesp, Jaboticabal-SP. The economical analyses were accomplished with prices of May and September of 2005. The total operational costs (COT) of the intercropping between crisp lettuce and roquette; looseleaf lettuce and roquette; and crisphead lettuce and roquette were from 14,8% to 95,6% highest to the of the singly cultivations of the cultures. The intercropping were shown profitable, the largest gross and liquid revenues happened in established intercropping with crisphead lettuce and roquette, in the Autumn-Winter, and with crisp lettuce and roquette, in the Spring.

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The experiment was carried in green house at Unesp, Jaboticabal-SP, in the period of August and November, with objective to evaluate the production cost and crisphead lettuce "Lucy Brown" profitability in monoculture and in intercropping with Japanese cucumber "Hokushin" in protected environment. The production cost determination was performed on total operational cost (COT) basis. The crisphead lettuce in monoculture estimated COT and in intercropping was R$ 657,57 and R$ 159,95/614,4 m(2) respectively. The productivities of 623 and 730 kg/614,4 m(2) in the experiments had provided gross incomes of 286,58 R$ and 335,80/614,4 m(2). It was verified a loss of R$ 370,99/614,4 m(2) in the crisphead lettuce monoculture and an operational profit of R$ 175,85/614,4 m(2), in protected environment when in intercropping with cucumber.

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The soil erodibility factor is represented by the integrated effect of processes that regulate the water infiltration, soil resistance and the transport of soil particles. Therefore, this study had as objective to estimate the soil erodibility in the Corrego do Tijuco watershed, São Paulo State, Brazil, for guiding farmers in decision making for application of techniques for soil conservation. The soil samples were collected in a regular spacing of 500 m, resulting in a total of 310 points in a 0-20.0 m depth in an area of approximately 8,000 ha. For the estimation of soil erodibility (K) was applied a mathematical model which takes into account the soil organic matter content, the sand and silt size fractions, the soil permeability, and the particle mean diameter for each point of soil sampling. Estimated values greater than 0.040 Mg ha h ha(-1) MJ(-1) mm(-1) were considered very high for this factor. In areas with greater degree of erodibility occurs the Argisols order predominance, that shows high susceptibility to the development of interrill erosion due to its remarkable performance in relation to textural gradient, thereby, the interrill erosion rates can be very intensive due to rapid saturation of upper horizon, increasing the erosion process. At the Latosols (Oxisols) areas were also observed high soil erodibility values that under inappropriate conditions of soil occupation, concentrating runoff of rain water, leading to appear deep gullies.

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In mechanized harvesting of seeds can occur losses, which reduce productivity, decrease the profitability and can do serious damage to the producer. The objective of this work was to evaluate the quantitative seed losses of Crotalaria juncea depending on the harvester speed and the rotation of the threshing cylinder. The experiment was conducted in the area of Education, Research and Production Unesp/Jaboticabal, using a entirely casualized design on factorial away 2x3, with two-speed displacement of the machine (1.8 and 3.2 km h(-1)) and three rotations of threshing cylinder (750, 800 and 850 rpm), with 4 repetitions. It was concluded that the measures relating to the development of the plants showed homogeneous behavior, indicating little dispersion of the data. It was observed that the rate of displacement of the harvester and rotation of the drum track not affected the losses in the plataform of cutting. The largest losses of crotalaria seeds occurred on the plataform of cutting the harvester machine, in the form of pods not harvested. It was observed that the total losses in the harvest of crotalaria seed were high, and the losses in pods on the soil, responsible for almost all the losses encountered.